The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. U of rochester biological supply ctr g8633 stock manager. Wide range of dna ladders and molecular weight markers from bioneer. This marker is ideal for the size determination of digested dna. Mix gently, but throughly, to ensure that the solution is homogeneous. The ladder is composed of fourteen chromatographypurified. Ladder profile and sizes of fermentas 1kb dna ladder sm0311. The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp. Thermo scientific ogeneruler 1 kb dna ladder, readytouse 25010,000 bp perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels with this 1 kb dna ladder, premixed with blue and yellow tracking dyes.
The blue dye migrates with 35 kb dna fragments in a 1% agarose gel and has an excitation peak of 424 nm. Sigmaaldrich offers a number of directload 1 kb dna ladder products. Thermo scientific generuler 1 kb dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 250 bp to 10,000 bp on agarose gels. This dna 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0. Researchcart360 does not make any representations or warranties as to the accuracy or timeliness of such information, which may be subject to change without notice. William smith general phone 2755731 molecular biology sales specialist.
The invitrogen 100 bp dna ladder is designed for sizing and. The dna ladder consists of 14 dna fragments and is provided with 6x tritrack dna loading dye. Hyperladder 1kb with 1% wv agarose gel, hyperladder 50bp with 1. Neb also offers a quickload version of this ladder with purple dye. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The dna ladder consists of 15 dna fragments and is provided with 6x tritrack dna loading dye. Follow the recommendations for loading described in the certificate of analysis of the dna laddersmarkers 0. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. Readytoload 1 kb dna ladder ranging from 500 to 10,000 bp. Generuler 1 kb plus dna ladder can be labeled radioactively with t4 polynucleotide kinase. A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes. Generuler 1 kb dna ladder 1250 25 x 50 g for 2500 applications, 0. The ladder is composed of fifteen chromatographypurified individual dna fragments in base pairs.
Description thermo scientific generuler 1 kb dna ladder is. Thermo scientific generuler 1 kb dna ladder, readytouse 11571595 print thermo scientific generuler 1 kb dna ladder, readytouse perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels. Readytoload 1 kb dna ladder ranging from 500 to 10,000. Plasmid dna digested in 5 min with fastdigest xhoi. In addition to these 12 bands, the ladder contains vector dna fragments that. All prices and stock availability are for informational purposes only. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination.
Thermo scientific generuler 1 kb dna ladder, readytouse, is recommended for sizing and approximate quantification of a wide range of doublestranded dna on agarose gels. Ladder profile and sizes of thermoscientific generuler 100 bp dna ladder plus sm0243, sm0323. The 100 bp dna ladder ranges from 100bp 1517 bp and is supplied with a free vial of. Thermo scientific generuler 1 kb plus dna ladder fisher. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. Thermo scientific fermentas fastdigest enzymes are an advanced line of restriction enzymes for rapid dna. The ladder is a mixture of chromatographypurified individual dna fragments. Thermo scientific generuler 1 kb plus dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 75 bp to 20,000 bp on agarose gels. Generuler 1 kb plus dna ladder 75 to 20,000 bp is recommended for sizing and approximate quantification of a wide range of doublestranded dna on agarose gels.
Page 14 material safety data sheet prepared in accordance with eu directive 91155eec printed. Thermo scientific fermentas fastdigest restriction enzymes. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye. Generuler 1 kb dna ladder, readytouse catalog number sm03, sm0314 pub. Dna ladder 100 bp and dna ladder 1kb marker for dna rna agarose gel electrophoresis. The 1kb plus dna ladder is ready to use, ranges from 25000250 base pairs, has intensities at 3k and 1k bp and is premixed with loading buffer. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and 1kb bands. Please have a look into the related application note for more information about ladder migration in different gel concentrations. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis.
A was added to eppendorf tubes to a final volume of 20. Thermo scientific generuler 1kb dna ladder, readytouse, is designed for sizing and approximate quantification of wide range double. Load on sdspolyacrylamide gel for minigels with a thickness of 0. Thermo scientific generuler 1 kb plus dna ladder is designed for sizing and quantification of dna fragments in agarose gels. How can i prepare working solution with 1 kb dna ladder. A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. Generuler 100 bp dna ladder thermo fisher scientific. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. Generuler 1 kb dna ladder, readytouse thermo fisher scientific.
Thermo scientific generuler 1 kb dna ladder, readytouse. Product information thermo scientific generuler 1 kb. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Thermo scientific generuler 1 kb dna ladder perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels with this mixture of chromatographypurified individual dna fragments. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. Thermo scientific generuler 100 bp dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 100 bp to 1,000 bp on agarose or polyacrylamide gels. Thermo scientific ogeneruler 1 kb dna ladder, readyto. The dna ladder consists of 10 dna fragments and is provided with 6x tritrack dna loading dye.
Ii total rna were analysed on a 1 % formaldehyde agarose gel. Product information thermo scientific generuler 1 kb dna. Generuler 1 kb dna ladder thermo fisher scientific. Comes supplied with 1 vial of gel loading dye, purple 6x, no sds. Generuler 1kb dna ladder, readytouse application of the substance the preparation. Contents amount storage sm03 generuler 1 kb dna ladder, readytouse 250 5 x 50 g for 500 applications, 0.
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